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Agilent 1200 hplc

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Agilent 1200 hplc Specification

Automation Grade
Fully automated

Number of Specimens
Up to 120-position autosampler

Mounting Type
Benchtop

Humidity
20% 85% RH (non-condensing)

Power Supply
100-240 V AC, 50/60 Hz

Test Range
Pressure: up to 600 bar (8700 psi)

Operating Voltage
100-240 V AC, 50/60 Hz

Specimen Size
Compatible with standard HPLC vials (2 mL, 4 mL, etc.)

Application
Analytical separation of chemical/biological samples

Accuracy
Flow accuracy: 1% or better

Features
Modular design, quaternary or binary pump, diode array detector, autosampler

Equipment Type
High Performance Liquid Chromatograph

Interface Type
LAN, RS232, USB (System dependent)

Resolution
Detector: 0.001 AU

Temperature
Ambient to 40C

Display Type
LCD display on some modules, PC interface

Measuring Range
Wavelength: 190 600 nm (detector dependent)

Frequency
50/60 Hz

Port Size
1/16 inch standard for fluidic connections

Response Time
< 1 sec for detector modules

Max Height
Approx. 45 cm (module dependent)

Usage
Pharmaceutical, chemical, biotechnology industries

Capacity
Up to 600 bar (system dependent)

Machine Weight
Approx. 50-65 kg (depending on configuration)

Test Speed
Up to 100 Hz sampling frequency

Control Mode
PC controlled via ChemStation or OpenLAB software

About Agilent 1200 hplc

Refurished Agilent 1200 HPLC with

Quatnery Pump

Degasser

Autosampler

Column Oven

VWD Detector

Software:Chemstation or EZ chrom

solvent tray

standard tubing and cables

Make: Agilent

Advanced Pumping Technology

The Agilent 1200 HPLC offers a choice between quaternary and binary gradient pumps, allowing users to handle complex gradients and optimize separations. Designed with stainless steel and PEEK materials, the system ensures chemical resistance and high-pressure durability up to 600 bar. This versatility supports a wide range of applications, from routine analyses to challenging separations.

Flexible Detection Capabilities

Choose from multiple detectors such as UV/VWD, DAD, FLD, RID, and ELSD. This flexibility covers analytical needs from UV-visible absorptions to fluorescence or refractive index measurements. Each detector delivers high sensitivity and rapid response rates, with noise levels typically below 10 AU at 254 nm, ensuring precise quantification.

Exceptional Sample Handling and Throughput

With a high-capacity autosampler capable of handling up to 120 standard HPLC vials, the Agilent 1200 is tailored for laboratories requiring efficient sample processing. Injection volumes can be set from 1100 L, catering to samples with varying volumes while achieving consistent results. Automated operations reduce manual interventions and increase lab productivity.

FAQs of Agilent 1200 hplc:

Q: How does the Agilent 1200 HPLC enable high sample throughput in analytical workflows?

A: The systems autosampler can process up to 120 samples automatically, significantly boosting throughput for analytical laboratories. This feature, combined with fully automated operation and rapid injection cycles, allows continuous, unattended runs and maximizes productivity.

Q: What are the primary benefits of using quaternary or binary gradient pumps in this equipment?

A: Quaternary and binary pumps provide flexibility in solvent selection, enabling precise gradient formation to tackle complex separation challenges. This adaptability enhances method development, ensures reproducibility, and supports a broader range of analytical applications.

Q: When is it advisable to use different detectors with the Agilent 1200 HPLC?

A: Detector choice depends on the samples properties and the analytical goals. Use UV/VWD or DAD for UV-active compounds, FLD for fluorescence analytes, RID for refractive index measurements, and ELSD for non-UV-absorbing components. Selecting the appropriate detector optimizes sensitivity and accuracy for your application.

Q: Where can the Agilent 1200 HPLC be best deployed for maximum efficiency?

A: This HPLC system is ideally suited for pharmaceutical, chemical, and biotechnology laboratories requiring high reliability, rapid analysis, and flexibility. Its benchtop modular design allows for seamless integration into both research and quality control environments.

Q: What is the process for controlling and monitoring the Agilent 1200 HPLC during operation?

A: The system is PC-controlled via Agilents ChemStation or OpenLab software, offering real-time monitoring, data acquisition, and method management. System modules may also feature LCD displays for direct status checks, and remote data interfaces support streamlined laboratory workflows.

Q: How does automatic leak detection and pressure limiting improve safety during HPLC runs?

A: Automatic leak detection quickly identifies fluid leaks, while programmable pressure limiters protect the system from overpressure events. These safety features minimize downtime, safeguard samples, and enhance operator safety throughout routine operation.

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